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p gsk3β  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p gsk3β
    P Gsk3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+gsk3%CE%B2/Phospho-GSK-3beta+(Ser9)+XP+Rabbit+mAb/pm42815069-98-54-55
    Average 96 stars, based on 1236 article reviews
    p gsk3β - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Targeting CXCR6 Disrupts β-Catenin Signaling and Enhances Sorafenib Response in Hepatocellular Carcinoma.
    Article Snippet: .. Membranes were blocked in 5% nonfat milk and incubated with primary antibodies against CXCR6 (Invitrogen/ThermoFisher, Waltham, MA, USA; #PA5-19936, 1:500), anti-β-catenin (Cell Signaling Technology, Danvers, MA, USA #9582; 1:500), pCREB (Cell Signaling Technology, #9198S; 1:500), GSK3β (Cell Signaling Technology, #9832; 1:1000), p-GSK3β (Cell Signaling Technology, #9336; 1:1000), and GAPDH (Invitrogen #39-8600; 1:1000) or Histone H2A.X (Thermofisher, Waltham, MA, USA #10856-1-AP; 1:1000) as loading controls. ..

    Article Title: Reduced IGF-1 signaling fails to limit Alzheimer’s disease progression in a novel rat model of IGF-1R haploinsufficiency
    Article Snippet: .. Membranes were blocked in 5% milk or BSA in TBST and then incubated with appropriate primary antibodies from Cell Signaling Technologies (Danvers, MA) against IGF-1R (Cell Signaling; #9750), InsR (Cell Signaling; #3025), p-GSK3β (Cell Signaling; #5558), total GSK3β (Cell Signaling; #12456), p-Tau(Ser202/Thr205) (Thermo Fisher AT8 #MN1020), total Tau (Cell Signaling; #46687), amyloid-beta (Cell Signaling; #8243), pS6 (catalog #5364), total S6 (Cell Signaling; #2217), p-Erk (Cell Signaling; #9101), total Erk (Cell Signaling; #4695), p-MAP2 (Ser136) (Cell Signaling #4541), Total MAP2 (Cell Signaling; #8707) overnight at 4 °C overnight with gentle agitation. .. Following a 1 h incubation with HRP-linked anti-rabbit secondary antibody (Cell Signaling; #7074P2), Clarity Western ECL (Biorad, #1705062) substrate was applied to membranes to visualize bands using the Biorad Chemidoc MP Imaging System to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA).

    Western Blot:

    Article Title: Cucurbitacin B attenuates LPS-induced skeletal muscle atrophy by inhibiting JAK2/STAT3 and suppressing proteasome activity.
    Article Snippet: .. Main primary antibodies used for Western blotting: MyoD (Novus Cat. no. NBP1–54153), MyHC (Abcam Cat. no. ab37484), p-IGF-1R (CST, Cat. no. 3918 T), IGF-1R (CST, Cat. no. 9750 T), IRS-1 (CST, Cat. no. 3407 T), p-Akt (CST, Cat. no. 4060),Akt (SC, Cat. no. Sc-5298), p-mTOR (CST, Cat. no. 2971), mTOR (SC, Cat. no. Sc-8319), p-GSK3β (CST, Cat. no. 5558), GSK3β (SC, Cat. no. Sc-53,931), p-p70S6K (CST, Cat. no. 9204), p70S6K (SC, Cat. no. Sc-8418), p-4E-BP1 (CST, Cat. no. 9451), 4E-BP1 (SC, Cat. no. Sc-6936), FoxO3a (CST, Cat. no. 12829S), Lamin B (Absin Cat. no. Abs131244), Atrogin-1 (Novus Cat. no. NBP1–79517), MuRF1 (SC, Cat. no. Sc-398,608), LC3B (CST, Cat. no. 43566 T), Beclin1 (CST, Cat. no. 3495), P62 (Abmart, Cat. no. T55546F), β-actin(Absin Cat. no. abs32001), p-STAT3 (Tyr 705) (CST, Cat. no. 9145), p-STAT3 (Ser 727) (CST, Cat. no. 9134), STAT3 (SC, Cat. no. Sc-8019), p-JAK1 (CST, Cat. no. 3331), JAK1 (CST, Cat. no. 3332), p-JAK2 (CST, Cat. no. 3776), JAK2 (CST, Cat. no. 34315), p-Src (CST, Cat. no. 2105), Src (CST, Cat. no. 2109), IL-1β (ABclonal,Cat. .. no. A16288),TNF-α (Abmart, Cat. no. PA2174F).

    Membrane:

    Article Title: Lithospermic Acid Promotes Osteoblast Proliferation and Differentiation through the Wnt/β-Catenin Signaling Pathway
    Article Snippet: Protein concentrations were measured with the BCA assay (PC0040, Solarbio), and equal protein quantities were resolved through SDS-PAGE before electrophoretic transfer to PVDF membranes. .. Following membrane blocking with 5% non-fat milk (1 h, RT), primary antibody incubations were performed at 4○C overnight targeting: PCNA (1:1000, #13110, CST), Ki67 (5 μg/mL, ab16667, Abcam), Cyclin D1 (1:1000, #2978, CST), cmyc (1:800, #13987, CST), RUNX2 (1:1000, ab192256, Abcam), OCN (1:800, bs-4917R, Bioss, Beijing, China), OPN (1:1000, ab218237, Abcam), OSX (1:800, bs-1110R, Bioss), BMP-2 (1:1000, ab284387, Abcam), Collagen I (1:500, bs-10423R, Bioss), Wnt-3a (1:1000, PA5-37320, Invitrogen), β-catenin (1:1000, #9562, CST), p-GSK3β (1:1000, #5558, CST), GSK-3β (1:1000, #9315, CST), and β-actin (1:10,000, bsm-63325R, Bioss). .. After TBST washes, HRP-conjugated secondary antibodies (1:5000, #7074, CST) were applied (1 h, RT).

    Blocking Assay:

    Article Title: Lithospermic Acid Promotes Osteoblast Proliferation and Differentiation through the Wnt/β-Catenin Signaling Pathway
    Article Snippet: Protein concentrations were measured with the BCA assay (PC0040, Solarbio), and equal protein quantities were resolved through SDS-PAGE before electrophoretic transfer to PVDF membranes. .. Following membrane blocking with 5% non-fat milk (1 h, RT), primary antibody incubations were performed at 4○C overnight targeting: PCNA (1:1000, #13110, CST), Ki67 (5 μg/mL, ab16667, Abcam), Cyclin D1 (1:1000, #2978, CST), cmyc (1:800, #13987, CST), RUNX2 (1:1000, ab192256, Abcam), OCN (1:800, bs-4917R, Bioss, Beijing, China), OPN (1:1000, ab218237, Abcam), OSX (1:800, bs-1110R, Bioss), BMP-2 (1:1000, ab284387, Abcam), Collagen I (1:500, bs-10423R, Bioss), Wnt-3a (1:1000, PA5-37320, Invitrogen), β-catenin (1:1000, #9562, CST), p-GSK3β (1:1000, #5558, CST), GSK-3β (1:1000, #9315, CST), and β-actin (1:10,000, bsm-63325R, Bioss). .. After TBST washes, HRP-conjugated secondary antibodies (1:5000, #7074, CST) were applied (1 h, RT).

    Gentle:

    Article Title: Reduced IGF-1 signaling fails to limit Alzheimer’s disease progression in a novel rat model of IGF-1R haploinsufficiency
    Article Snippet: .. Membranes were blocked in 5% milk or BSA in TBST and then incubated with appropriate primary antibodies from Cell Signaling Technologies (Danvers, MA) against IGF-1R (Cell Signaling; #9750), InsR (Cell Signaling; #3025), p-GSK3β (Cell Signaling; #5558), total GSK3β (Cell Signaling; #12456), p-Tau(Ser202/Thr205) (Thermo Fisher AT8 #MN1020), total Tau (Cell Signaling; #46687), amyloid-beta (Cell Signaling; #8243), pS6 (catalog #5364), total S6 (Cell Signaling; #2217), p-Erk (Cell Signaling; #9101), total Erk (Cell Signaling; #4695), p-MAP2 (Ser136) (Cell Signaling #4541), Total MAP2 (Cell Signaling; #8707) overnight at 4 °C overnight with gentle agitation. .. Following a 1 h incubation with HRP-linked anti-rabbit secondary antibody (Cell Signaling; #7074P2), Clarity Western ECL (Biorad, #1705062) substrate was applied to membranes to visualize bands using the Biorad Chemidoc MP Imaging System to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA).



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    Inhibition of ITGB1 downregulates the β-catenin/c-Myc pathway in HUVECs. (A) ITGB1 gene expression after HUVECs were transfected with 20 nM ITGB1 siRNA1, siRNA2 or siRNA negative control for 6 h, and cultured for another 48 h. The gene expressions of (B) ITGB1-DT, (C) EZH2 and (D) c-Myc were assessed using reverse transcription-quantitative PCR following transfection with ITGB1 siRNA. (E) The protein expression levels of ITGB1, TCF1, EZH2, <t>c-Myc,</t> <t>p-GSK3β,</t> GSK3β, p-β-catenin and β-catenin were evaluated using western blotting following transfection with ITGB1 siRNA. (F) Semi-quantification of western blotting results. p-GSK3β and p-β-catenin were normalized to the levels of total GSK3β or β-catenin, respectively. (G) Cell viability was analyzed using a Cell Counting Kit-8 assay. n=3; *P<0.05 and **P<0.01 compared with siRNA control group. HUVECs, human umbilical vein endothelial cells; ITGB1, integrin β1; ITGB1-DT, integrin subunit β1 divergent transcript; EZH2, histone-lysine N-methyltransferase EZH2; siRNA, small interfering RNA; TCF1, T-cell factor 1; p-, phosphorylated.
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    Inhibition of ITGB1 downregulates the β-catenin/c-Myc pathway in HUVECs. (A) ITGB1 gene expression after HUVECs were transfected with 20 nM ITGB1 siRNA1, siRNA2 or siRNA negative control for 6 h, and cultured for another 48 h. The gene expressions of (B) ITGB1-DT, (C) EZH2 and (D) c-Myc were assessed using reverse transcription-quantitative PCR following transfection with ITGB1 siRNA. (E) The protein expression levels of ITGB1, TCF1, EZH2, <t>c-Myc,</t> <t>p-GSK3β,</t> GSK3β, p-β-catenin and β-catenin were evaluated using western blotting following transfection with ITGB1 siRNA. (F) Semi-quantification of western blotting results. p-GSK3β and p-β-catenin were normalized to the levels of total GSK3β or β-catenin, respectively. (G) Cell viability was analyzed using a Cell Counting Kit-8 assay. n=3; *P<0.05 and **P<0.01 compared with siRNA control group. HUVECs, human umbilical vein endothelial cells; ITGB1, integrin β1; ITGB1-DT, integrin subunit β1 divergent transcript; EZH2, histone-lysine N-methyltransferase EZH2; siRNA, small interfering RNA; TCF1, T-cell factor 1; p-, phosphorylated.
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    Image Search Results


    A. Western Blot analysis of P-GSK3β (S9), GSK3β, P-C-MYC T58, C-MYC & β-ACTIN in untreated control as well as MK-1775 (0.5μM & 1μM) treated OE33, SK-GT4 & OE19 cell lines. B. Western Blot analysis of P-GSK3β (S9), GSK3β, P-C-MYC T58, C-MYC & β-ACTIN in control siRNA and WEE1 siRNA treated OE33, SK-GT4 & OE19 cell lines. C. PLA to visualize C-MYC and GSK3β interaction (red spots) in untreated Control & MK-1775 treated OE19, OE33, and SK-GT4 cell lines. Captured at 40X magnification. D. Western blot analysis of WEE1, P-CDC2 Y15, C-MYC, P-GSK3β (S9), GSK3β, and β-ACTIN in empty vector as well as WEE1-CDS vector transfected OE33, SK-GT4, and OE19 cell lines. E. Western blot analysis of WEE1, P-CDC2 Y15, CDC2, C-MYC, and β-ACTIN in empty vector, WEE1-CDS-WT, and WEE1-CDS-K328A kinase dead mutant vector transfected SK-GT4 cells.

    Journal: Cancer letters

    Article Title: WEE1 Stabilizes MYC to Promote Therapeutic Resistance in Esophageal Adenocarcinoma

    doi: 10.1016/j.canlet.2026.218418

    Figure Lengend Snippet: A. Western Blot analysis of P-GSK3β (S9), GSK3β, P-C-MYC T58, C-MYC & β-ACTIN in untreated control as well as MK-1775 (0.5μM & 1μM) treated OE33, SK-GT4 & OE19 cell lines. B. Western Blot analysis of P-GSK3β (S9), GSK3β, P-C-MYC T58, C-MYC & β-ACTIN in control siRNA and WEE1 siRNA treated OE33, SK-GT4 & OE19 cell lines. C. PLA to visualize C-MYC and GSK3β interaction (red spots) in untreated Control & MK-1775 treated OE19, OE33, and SK-GT4 cell lines. Captured at 40X magnification. D. Western blot analysis of WEE1, P-CDC2 Y15, C-MYC, P-GSK3β (S9), GSK3β, and β-ACTIN in empty vector as well as WEE1-CDS vector transfected OE33, SK-GT4, and OE19 cell lines. E. Western blot analysis of WEE1, P-CDC2 Y15, CDC2, C-MYC, and β-ACTIN in empty vector, WEE1-CDS-WT, and WEE1-CDS-K328A kinase dead mutant vector transfected SK-GT4 cells.

    Article Snippet: C-MYC, p-C-MYC T58, P-GSK3β S9, GSK3β, PARP, CL-PARP, Caspase 3, Cl-Caspase 3, HA tag, CD133, SOX9, SOX2, CD44, OCT4, Nanog and Ki-67 antibodies were obtained from Cell Signaling Technologies (Danvers, MA).

    Techniques: Inhibition, Knockdown, Phospho-proteomics, Western Blot, Control, Plasmid Preparation, Transfection, Mutagenesis

    Inhibition of ITGB1 downregulates the β-catenin/c-Myc pathway in HUVECs. (A) ITGB1 gene expression after HUVECs were transfected with 20 nM ITGB1 siRNA1, siRNA2 or siRNA negative control for 6 h, and cultured for another 48 h. The gene expressions of (B) ITGB1-DT, (C) EZH2 and (D) c-Myc were assessed using reverse transcription-quantitative PCR following transfection with ITGB1 siRNA. (E) The protein expression levels of ITGB1, TCF1, EZH2, c-Myc, p-GSK3β, GSK3β, p-β-catenin and β-catenin were evaluated using western blotting following transfection with ITGB1 siRNA. (F) Semi-quantification of western blotting results. p-GSK3β and p-β-catenin were normalized to the levels of total GSK3β or β-catenin, respectively. (G) Cell viability was analyzed using a Cell Counting Kit-8 assay. n=3; *P<0.05 and **P<0.01 compared with siRNA control group. HUVECs, human umbilical vein endothelial cells; ITGB1, integrin β1; ITGB1-DT, integrin subunit β1 divergent transcript; EZH2, histone-lysine N-methyltransferase EZH2; siRNA, small interfering RNA; TCF1, T-cell factor 1; p-, phosphorylated.

    Journal: Molecular Medicine Reports

    Article Title: miRNA-29a inhibits the proliferation of HUVECs by regulating the ITGB1/β-catenin/c-Myc pathway

    doi: 10.3892/mmr.2026.13825

    Figure Lengend Snippet: Inhibition of ITGB1 downregulates the β-catenin/c-Myc pathway in HUVECs. (A) ITGB1 gene expression after HUVECs were transfected with 20 nM ITGB1 siRNA1, siRNA2 or siRNA negative control for 6 h, and cultured for another 48 h. The gene expressions of (B) ITGB1-DT, (C) EZH2 and (D) c-Myc were assessed using reverse transcription-quantitative PCR following transfection with ITGB1 siRNA. (E) The protein expression levels of ITGB1, TCF1, EZH2, c-Myc, p-GSK3β, GSK3β, p-β-catenin and β-catenin were evaluated using western blotting following transfection with ITGB1 siRNA. (F) Semi-quantification of western blotting results. p-GSK3β and p-β-catenin were normalized to the levels of total GSK3β or β-catenin, respectively. (G) Cell viability was analyzed using a Cell Counting Kit-8 assay. n=3; *P<0.05 and **P<0.01 compared with siRNA control group. HUVECs, human umbilical vein endothelial cells; ITGB1, integrin β1; ITGB1-DT, integrin subunit β1 divergent transcript; EZH2, histone-lysine N-methyltransferase EZH2; siRNA, small interfering RNA; TCF1, T-cell factor 1; p-, phosphorylated.

    Article Snippet: The membranes were blocked with 5% skimmed milk for 1 h at room-temperature and incubated with the following primary antibodies overnight at 4°C: ITGB1 (cat. no. 12594-1-AP; Proteintech Group, Inc.), T-cell factor 1 (TCF1; cat. no. 14464-1-AP; Proteintech Group, Inc.), EZH2 (cat. no. ab307646; Abcam), c-Myc (cat. no. 67447-1-Ig; Proteintech Group, Inc.), phosphorylated (p-)GSK3β (cat. no. AF2016; Affinity Biosciences), GSK3β (cat. no. AF5016; Affinity Biosciences), p-β-catenin (cat. no. DF2989; Affinity Biosciences), β-catenin (cat. no. 17565-1-AP; Proteintech Group, Inc.), β-actin (cat. no. 66009-1-Ig; Proteintech Group, Inc.), OGT (cat. no. ab177941; Abcam) and OGA (cat. no. DF8436; Affinity Biosciences).

    Techniques: Inhibition, Gene Expression, Transfection, Negative Control, Cell Culture, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Cell Counting, Control, Small Interfering RNA

    miR-29a downregulates the β-catenin/c-Myc pathway in HUVECs by targeting ITGB1. HUVECs were transfected with 20 µg/ml miR-29a mimics, miR-29a inhibitor or negative control for 6 h and cultured for another 48 h. (A) The protein expression levels of OGT, OGA, ITGB1, TCF1, c-Myc, p-GSK3β, GSK3β, p-β-catenin and β-catenin were assessed using western blotting and (B) semi-quantified. p-GSK3β and p-β-catenin were normalized to the levels of total GSK3β or β-catenin, respectively. (C) Cell viability was analyzed using a Cell Counting Kit-8 assay. The interaction between miR-29a and ITGB1 was validated using (D) TargetScan and (E) via dual-luciferase reporter assay (**P<0.01 compared with miR NC + ITGB1 WT group). (F) A schematic figure illustrating the interactions among miR-29a, ITGB1 and the c-Myc pathway. n=3; *P<0.05 and **P<0.01 compared with miRNA-NC group. miR/miRNA, microRNA; HUVECs, human umbilical vein endothelial cells; ITGB1, integrin β1; OGT, O-linked N-acetylglucosamine transferase 110 kDa subunit; OGA, β-N-acetylglucosaminidase; TCF1, T-cell factor 1; p- phosphorylated; hsa-miR, human microRNA; NC, negative control; WT, wild-type; Mut, mutant.

    Journal: Molecular Medicine Reports

    Article Title: miRNA-29a inhibits the proliferation of HUVECs by regulating the ITGB1/β-catenin/c-Myc pathway

    doi: 10.3892/mmr.2026.13825

    Figure Lengend Snippet: miR-29a downregulates the β-catenin/c-Myc pathway in HUVECs by targeting ITGB1. HUVECs were transfected with 20 µg/ml miR-29a mimics, miR-29a inhibitor or negative control for 6 h and cultured for another 48 h. (A) The protein expression levels of OGT, OGA, ITGB1, TCF1, c-Myc, p-GSK3β, GSK3β, p-β-catenin and β-catenin were assessed using western blotting and (B) semi-quantified. p-GSK3β and p-β-catenin were normalized to the levels of total GSK3β or β-catenin, respectively. (C) Cell viability was analyzed using a Cell Counting Kit-8 assay. The interaction between miR-29a and ITGB1 was validated using (D) TargetScan and (E) via dual-luciferase reporter assay (**P<0.01 compared with miR NC + ITGB1 WT group). (F) A schematic figure illustrating the interactions among miR-29a, ITGB1 and the c-Myc pathway. n=3; *P<0.05 and **P<0.01 compared with miRNA-NC group. miR/miRNA, microRNA; HUVECs, human umbilical vein endothelial cells; ITGB1, integrin β1; OGT, O-linked N-acetylglucosamine transferase 110 kDa subunit; OGA, β-N-acetylglucosaminidase; TCF1, T-cell factor 1; p- phosphorylated; hsa-miR, human microRNA; NC, negative control; WT, wild-type; Mut, mutant.

    Article Snippet: The membranes were blocked with 5% skimmed milk for 1 h at room-temperature and incubated with the following primary antibodies overnight at 4°C: ITGB1 (cat. no. 12594-1-AP; Proteintech Group, Inc.), T-cell factor 1 (TCF1; cat. no. 14464-1-AP; Proteintech Group, Inc.), EZH2 (cat. no. ab307646; Abcam), c-Myc (cat. no. 67447-1-Ig; Proteintech Group, Inc.), phosphorylated (p-)GSK3β (cat. no. AF2016; Affinity Biosciences), GSK3β (cat. no. AF5016; Affinity Biosciences), p-β-catenin (cat. no. DF2989; Affinity Biosciences), β-catenin (cat. no. 17565-1-AP; Proteintech Group, Inc.), β-actin (cat. no. 66009-1-Ig; Proteintech Group, Inc.), OGT (cat. no. ab177941; Abcam) and OGA (cat. no. DF8436; Affinity Biosciences).

    Techniques: Transfection, Negative Control, Cell Culture, Expressing, Western Blot, Cell Counting, Luciferase, Reporter Assay, Mutagenesis